, Biorad). The mice were perfused and the cervical spinal cords and brains dissected and sequentially dehydrated in 10, 20, and 30% sucrose. Tissues were then embedded in optimum cutting temperature compound , frozen, sectioned and mounted on slides. Slides were warmed at 37°C for 30 min and OCT was washed away with PBS. Sections were then blocked at room temperature with 2.5% bovine serum albumin in PBS with 0.
Coronal section of the injured spinal cord stained using GFAP and NeuN antibodies . DAPI stained nuclei. Scale bar, 200 μm.
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