The SYNGAP1 3′UTR Variant in ALS Patients Causes Aberrant SYNGAP1 Splicing and Dendritic Spine Loss by Recruiting HNRNPK

3′UTR sequence was amplified from the plasmids produced in the 3′ rapid amplification of cDNA ends of wild-type iPSC-derived motor neurons. The mutation was introduced using the PrimeSTAR Mutagenesis Basal kit , according to the manufacturer's instructions.

The immunofluorescent signal intensity was measured by ZEN software . The quantification of spine number was performed as described previously . Regions of dendrites located 30–100 µm from the cell soma that did not intersect with other dendrites were selected. The numbers of spines, labeled with Alexa Fluor 488-conjugated phalloidin, were counted over a region of 20 µm in length. The number of spines was counted independently, without sample information.

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