). Serum samples for the antibody detection and laboratory endpoints were collected at “baseline” antedating reactive de novo changes and at “follow-up” . Lesion-dependent aspects were evaluated based on the International Standards for Neurological Classification of SCI at baseline.
Demographic baseline data and clinical information on infections, medication, red blood transfusions, and fluid resuscitation were collected using paper case report forms.The samples for TBA and DRG assays were provided by Charité-Universitätsmedizin Berlin, Department of Neurology in a blinded manner using a 6-digit pseudonym as identifier without any accompanying clinical information.
Because we used rat cells and tissues, putative binding of heterophilic antibodies was prevented using blocking strategies including 10% donkey serum or fetal calf serum incubation. The results of TBA and DRG assessments were sent to Charité-Universitätsmedizin Berlin, Department of Neurology for unblinding and statistical analysis.Adult Sprague Dawley rats were euthanized and spinal cords removed and fixed in 4% paraformaldehyde for 4 hours at 4°C.
OhioState Thanks for sharing this great work from OSUWexMed! We sent out an update to the press release you are linking to; here is the updated, corrected version: