The Smc5/6 complex is a DNA loop-extruding motor - Nature

. Intensity time trace of Smc5/6 depicted in showing one-step bleaching. Probability density functions of fluorescence intensities for from Alexa647 signal on labeled Smc5/6 and the background signal . Calculated propability of observing single bleaching steps as a function of dimer fraction given that we experimentally only observe one-step and two-step bleaching events. Red line shows the experimentally observed value shown in Fig.

, indicating that 90% of events are likely come from single Smc5/6 complexes Example kymographs of Smc5/6 on DNA in the presence of ATP showing diffusive and immobile characteristics. Example kymograph of Smc5/6 remaining bound to DNA in the presence of AMP-PNP. Mean square displacements of Smc5/6 complexes displaying directed motion corresponding to Fig., diffusive motion extracted from the kymograph shown in , and immobile behaviour corresponding to the kymograph in .

Mass distributions of pentameric and hexameric Smc5/6 complexes in different conditions, including no ligand , in the presence of DNA , ATP hydrolysis-deficient mutant with ATP and DNA , hexameric Smc5/6 with ATP and DNA . The identified peaks exhibit mass distributions close to the molecular weight of pentamer , single hexamer and dimer of hexamer . The inset panels show the zoom-in peak corresponding to the dimer of hexamers.

Car Car Latest News, Car Car Headlines

Similar News:You can also read news stories similar to this one that we have collected from other news sources.

Driving Genetic Discovery: New Insights Into DNA Replication’s Molecular MotorDNA replication is the process whereby cells make an exact copy of their DNA before cell division. A key part of the intricate DNA replication machinery is a molecular motor called CMG, which has the vital task of separating the two strands of the DNA double helix so that they can be copied. An inte
Source: SciTechDaily1 - 🏆 84. / 68 Read more »

A somato-cognitive action network alternates with effector regions in motor cortex - NatureFunctional MRI studies across ages show that the classic homunculus of the motor cortex in humans is in fact discontinuous, alternating with action control-linked regions termed the somato-cognitive action network.
Source: Nature - 🏆 64. / 68 Read more »